Schistosoma. h (ova)

KOH Aspergillus spp

🔬Amastigoute of Leishmania parasite

🔬Amastigoute of Leishmania parasite

Gametocyte of P. F

Gametocyte of P. F

🔬schizont malaria

CLL - Small lymphocytes with smudge cells at
arrows

CLL -Albumin smear: same patient as
presented in

Addition of albumin to blood before smear
preparation stabilizes CLL cells, decreasing the
formation of smudge cells and allows for accurate
cell classification

CLL- Small lymphocytes with smudge cell

CLL-BM demonstrating many lymphocytes

CLL- MORPHOLOGY
Peripheral Blood: Mature-appearing lymphocytes with round nuclei and block-type chromatin; inconspicuous
nucleoli, scant cytoplasm; homogeneous appearance within a given patient; lymphocytes more fragile than
normal, leading to “smudge” cells
• Absolute sustained lymphocytosis
• Normocytic normochromic anemia (approximately 10% of patients develop an autoimmune hemolytic
anemia)
• Thrombocytopenia
Bone Marrow: 30% or more lymphocytes
IMMUNOPHENOTYPE
CD20+, CD19+, CD5+, CD23+

Peripheral blood (PB) film
in CLL showing two mature
lymphocytes and one smear cell.
Nuclear chromatin is condensed
and each cell contains a barely
detectable nucleolus. May–
Grünwald–Giemsa

PB film from a patient
with CLL showing: (a) a smear
cell and four lymphocytes
containing globular
immunoglobulin inclusions

immunocytochemistry showing
expression of immunoglobulin
M.

PB film from a patient with
CLL showing the negative image of
an immunoglobulin crystal.

PB film in CLL complicated
by autoimmune haemolytic
anaemia showing one mature
lymphocyte, one smear cell,
spherocytes and polychromatic
macrocytes