الي عندو فكرة الجهاز دا Print كيف يكلمنا اذا ممكن😊👇

@groupmls_bot

#Mycology 💜👇

#Microbiology 💜👇

Procedure for primary culture from Trans-Isolate (T-I) medium Remove the aluminum cap of Trans-isolate medium with forceps Wipe the rubber stopper with a 70% alcohol swab (do not use povidone-iodine as it may be carried into the medium by the passing needle, thus inhibiting the growth of bacteria.) Aseptically inoculate 0.5-1.0 ml of the CSF into the T-I medium with the use of syringe Incubate the inoculated T-I medium at 35-37°C with ~5% CO2 (or in a candle-jar) overnight or until transport is possible If the T-I medium can be transported to a microbiology laboratory the same day of inoculation, do not vent the T-I bottle until it arrives in the receiving laboratory. Upon arrival, wipe the rubber stopper with 70% alcohol, insert a venting needle into the T-I bottle, incubate at 35-37°C with ~5% CO2 (or in a candle-jar), and observe daily for turbidity in the liquid phase for up to 7 days. Prior to subculture, remove the venting needle and wipe the rubber stopper with 70% alcohol. Use a sterile needle and syringe to transfer 50-100 µl of the liquid portion of the T-I medium onto both a Blood agar Plate (BAP) and Chocolate agar (CAP) for primary culture. Approximately 50-100 µl is used to streak each plate. To streak two plates, draw approximately 100-200 µl with the syringe at one time to minimize the possibility of contaminating the T-I medium. Streak the BAP and CAP for isolation, incubate the plates at 35-37°C with ~5% CO2 (or in a candle-jar), and examine the plates daily for up to 72 hours. If no growth is observed, subculture the T-I medium again on day 4 and day 7. Isolates should always be inspected for purity of growth by looking at colony morphology before any testing is performed. If any contamination is seen, cultures should be re-streaked to ensure purity prior to testing.

يادكاترة ببحث عن الكتاب دا 👇

kuby in Immunology

لْـۆ في شخص عندو يرسلو لينا هنا 👇💙
@groupmls_bot

kuby in Immunology💜👇

#شكرا الدكتور/ة الي رسلو لي 💜

♻Biochemistry

💭Metabolism carbohydrates 1

#بالعربي كدا نقصد شنو بال Metabolism carbohydrates ؟؟
نقصد ال life cycle 🙂
بنقصد قصة حياة الكربوهيدرات مثلا من لما دخلت ال body لغاية ما اتكسرت وطلعت لينا energy 💜 (الي هم ال anabolsim و ال catabolsim)


#علشان نحصل على ال energy في النهاية يعني بندخل في كم pathway وكم steps 💜 وبتبدأ من:
💡digestion and absorption of CHO
(ال CHO اختصار لكربوهيدرات 💜)

💡formation of acetyle CoA

💡anabolsim and catabolsim

💡formation of ATP


⭕العملية دي كلها اسمها ال Glycolysis : وهي العملية البتم فيها تناول الكربوهيدرات وتكسيرها في سلسلة من التفاعلات الكيميائية عن طريق انزيمات لوحدات صغيرة تتمثل في الجلكوز


💭عشان نفهم السلسلة من التفاعلات دي عبارة شنو عن. substrate 1 وبتلقى االانزيم الأول (البنسميهو ال key enzyme لانو هو الببدأ الشغل) بتتحول إلى. substrate 2 الي حتلقى enzyme 2 ويحولها لي substrate 3 الي حتلقى enzyme 3 وكدا القصة مكملة لغاية ما حنصل الsubstrate الاخيرة البنسميها ال.end product 💜

🌸🌸🌸🌸🌸🌸

When glucose enters a cell, the enzyme hexokinase (or glucokinase, in the liver) rapidly adds a phosphate to convert it into glucose-6-phosphate. A kinase is a type of enzyme that adds a phosphate molecule to a substrate (in this case, glucose, but it can be true of other molecules also). This conversion step requires one ATP and essentially traps the glucose in the cell, preventing it from passing back through the plasma membrane, thus allowing glycolysis to proceed. It also functions to maintain a concentration gradient with higher glucose levels in the blood than in the tissues. By establishing this concentration gradient, the glucose in the blood will be able to flow from an area of high concentration (the blood) into an area of low concentration (the tissues) to be either used or stored. Hexokinase is found in nearly every tissue in the body. Glucokinase, on the other hand, is expressed in tissues that are active when blood glucose levels are high, such as the liver. Hexokinase has a higher affinity for glucose than glucokinase and therefore is able to convert glucose at a faster rate than glucokinase. This is important when levels of glucose are very low in the body, as it allows glucose to travel preferentially to those tissues that require it more.

🌸🌸🌸🌸🌸🌸🌸


#يتبع …💜

#سؤال …إضافة … 💜👇

@groupmls_bot

#عهــــد_فتح_اللّـہ̣̥ 🔬👩‍🔬

https://www.tg-me.com/MLSbeautifulworld

#glycolysis equation💜

Glucose+2ATP+2NAD
​+
​​ +4ADP+2P
​i
​​ →Pyruvate+4ATP+2NADH+2H

Glycogenolysis: The breakdown or hydrolysis of glycogen in the liver (as well as kidneys, muscle and brain) into glucose which is released into the bloodstream.

Glycolysis: The oxidative breakdown or metabolism of glucose to produce energy

Glycogenesis: The conversion of excess glucose into glycogen as a cellular storage mechanism.

Gluconeogenesis: The reversal of glycogenolysis whereby glucose is synthesized within the body from non-carbohydrate substances, such as pyruvate, lactate, glycerol, and certain amino acids.

مشاهدة "Carbohydrate Metabolism" على YouTube
https://youtu.be/la54wsQClRc

💌ممكن الشباب الي معانا أي حد عندو شيتات لل chemistry او biochemistry يشاركهَا معانا وجزاكم الله خيرا 💜

#عهــــد_فتح_اللّـہ̣̥